IHC image of CSB-PA009500OA01HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
GLI2 Antibody
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, A549 whole cell lysate All lanes: GLI2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 168, 134, 132, 89, 87 kDa Observed band size: 168 kDa
Supplier Page from CUSABIO Technology LLC for GLI2 Antibody